Regulation of Malate Oxidation in Isolated Mung Bean Mitochondria
- 1 September 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 58 (3) , 433-437
- https://doi.org/10.1104/pp.58.3.433
Abstract
To investigate the relationship between malate oxidation and subsequent cycle reactions, the effects of oxaloacetate, pyruvate, and thiamine pyrophosphate on malate oxidation in mung bean (Phaseolus aureus cv. ''Jumbo'') hypocotyl mitochondria were quantitatively examined. Malate oxidation was optimally stimulated by addition of pyruvate and thiamine pyrophosphate, whose addition lowered the apparent Km for malate from 5 mM to 0.1 mM. Intermediate analysis showed that the stimulatory effect was correlated with removal of oxaloacetate to citrate. Oxaloacetate added alone was shown not to be metabolized until addition of pyruvate and thiamine pyrophosphate; then oxaloacetate was converted in part to pyruvate and citrate. Malate oxidation in mung bean mitochondria apparently is subject to control by oxalocetate levels, which are primarily determined by the results of the activities of malate dehydrogenase, citrate synthase and pyruvate dehydrogenase.This publication has 13 references indexed in Scilit:
- Regulation of Malate Oxidation in Isolated Mung Bean MitochondriaPlant Physiology, 1976
- The Oxidation of Malate and Exogenous Reduced Nicotinamide Adenine Dinucleotide by Isolated Plant MitochondriaPlant Physiology, 1974
- Pathways for the Oxidation of Malate and Reduced Pyridine Nucleotide by Wheat MitochondriaEuropean Journal of Biochemistry, 1973
- Oxalacetate control of Krebs cycle oxidations in purified plant mitochondriaBiochemical and Biophysical Research Communications, 1972
- The Oxidation of Malate by Isolated Plant MitochondriaEuropean Journal of Biochemistry, 1972
- The Oxidation of Malate by Mitochondria Isolated from Cauliflower BudsEuropean Journal of Biochemistry, 1970
- Metabolic Processes in Cytoplasmic Particles of the Avocado Fruit. IX. The Oxidation of Pyruvate and Malate during the Climacteric CyclePlant Physiology, 1967
- Preparation and Properties of Sweet Potato MitochondriaPlant Physiology, 1963
- Metabolic Processes in Cytoplasmic Particles of the Avocado Fruit. III. The Operation of the Tricarboxylic Acid Cycle.Plant Physiology, 1957
- Cofactor Requirements for Oxidation of Alpha-Keto Acids by Sweet Potato Mitochondria.Plant Physiology, 1956