NICKEL-63-CONSTITUENTS IN RENAL CYTOSOL OF RATS AFTER INJECTION OF NI-63 CHLORIDE

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  • 1 January 1981
    • journal article
    • research article
    • Vol. 11  (6) , 488-496
Abstract
Chromatographic and electrophoretic fractionations of 63Ni-constituents were performed on renal cytosol samples from rats that were killed following administration of 63NiCl2. At 0.5-1 h after i.v. injection of 63NiCl2 (0.1-0.5 .mu.mol/kg body wt, n = 6), renal cytosol contained 11% (SE .+-. 2%) of the 63Ni-dose. An average of 68% (SE .+-. 4%) of 63Ni in these cytosol samples was associated with low MW components (< 2000). The remainder was bound to 5 macromolecular constituents, with MW of > 130,000 (peak A), .apprx. 70,000 (peak B), .apprx. 55,000 (peak C), .apprx. 30,000 (peak D) and .apprx. 10,000 (peak E), based on column chromatography on Sephadex G-200. The 63Ni that was present in peaks A-E averaged, respectively, 1.8, 10.7, 7.5, 10.0 and 1.6% of total 63Ni in renal cytosol. At 1-4 h after i.m. injection of 63NiCl2 (100 .mu.mol/kg body wt, n = 17), renal cytosol contained 2.2% (SE .+-. 0.1%) of the 63Ni-dose; ultrafiltrable 63Ni averaged 57% (SE .+-. 3%) of total 63Ni in renal cytosol. Macromolecular 63Ni-constituents in these samples were separated by electrophoresis in agarose gel and visualized by autoradiography. Peak D (MW .apprx. 30,000) the most prominent of the 5 63Ni-constituents. Renal cytosol of 63NiCl2-treated rats contained 5 macromolecular 63Ni-constituents that may be involved in renal uptake, transport, storage and excretion of 63Ni.