Hydroxystreptomycin Production and Resistance in Streptomyces glaucescens

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Abstract
The wild-type strain of S. glucescens produces hydroxystreptomycin and shows an inherent natural resistance to streptomycin group aminoglycoside antibiotics. Cell-free extracts of the wild-type strain inactivated streptomycin, hydroxystreptomycin and dihydrostreptomycin in the presence of ATP. The phosphotransferase did not inactivate other aminoglycoside antibiotics, including spectinomycin. Mutant strains which were highly sensitive to streptomycin group aminoglycosides, had no measurable phosphotransferase activity and were unable to form detectable amounts of hydroxystreptomycin, were isolated. This suggests a correlation between phosphotransferase activity, streptomycin resistance and hydroxystreptomycin formation or defects in more than 1 gene in the strS mutant strains tested.