The Effect of Indomethacin on the Motility of Isolated Sheep Ureters

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Abstract
The possible spasmolytic effect of indomethacin was studied on isolated sheep ureteral preparations. Ureteral ring preparations suspended in an organ bath exhibited long lasting rhythmic contractions quite similar to the in vivo situation. This spontaneous motility was unaffected by hyoscine, phenoxybenzamine, propranolol, mepyramine and methsergide. Additions of indomethacin resulted in a dose dependent reduction in amplitude and frequency of the contractions, the threshold value being 10-8 M. Washing reestablished motility and both PGE2 and PGF2.alpha. were powerful stimulators of rhythmic contractions. In vitro rhythmic activity of the ureter evidently is dependent on endogeneous PG-synthesis and the cyclooxygenase inhibitor indomethacin dose-dependently blocks this.