Failure of 1,25 Dihydroxycholecalciferol [1,25-(OH)1-D3] to Modify Cyclic AMP Levels in Parathyroid Hormone-Treated and Untreated Bone Cells (1)
- 1 January 1976
- journal article
- Published by Taylor & Francis in Endocrine Research Communications
- Vol. 3 (2) , 157-166
- https://doi.org/10.3109/07435807609052930
Abstract
Treatment with 1,25-(OH)2-D3 (1 ng/ml - 25 ng/ml) for periods ranging from 2.5 min. to 60 min. did not alter cyclic AMP levels in bone cells isolated from periosteum-free rat calvaria, or in cells isolated from rat periosteal tissues. 1,25-(OH)2-D3 failed to modify the acute increases in cyclic AMP elicited by PTH (10 ng/ml -1 ug/ml). Two separate 1,25-(OH)2-D3 preparations, biologically active in other systems, were ineffective under a wide variety of experimental conditions. These results suggest that 1,25-(OH)2-D3 is not an acute modulator of cyclic AMP metabolism in PTH-treated and untreated bone cells.Keywords
This publication has 11 references indexed in Scilit:
- Effects of Parathyroid Hormone on the Accumulation of Cyclic AMP in Bone of Vitamin D-Deficient RatsEndocrinology, 1975
- Target cells in bone for parathormone and calcitonin are different: enrichment for each cell type by sequential digestion of mouse calvaria and selective adhesion to polymeric surfaces.Proceedings of the National Academy of Sciences, 1975
- Early effect of 25-hydroxycholecalciferol (25-OH-D3) and 1,25-dihydroxycholecalciferol (1,25-(OH)2-D3) on the ability of parathyroid hormone (PTH) to elevate cyclic AMP of intact bone cellsBiochemical and Biophysical Research Communications, 1975
- Separation of parathyroid hormone and calcitonin-sensitive cells from non-responsive bone cellsNature, 1974
- The role of vitamin D metabolites in bone resorptionCalcified Tissue International, 1973
- Cyclic 3′5′ Adenosine Monophosphate in Isolated Bone Cells: Response to Low Concentrations of Parathyroid Hormone12 12Endocrinology, 1973
- 1,25-Dihydroxycholecalciferol: A Potent Stimulator of Bone Resorption in Tissue CultureScience, 1972
- COMPETITIVE PROTEIN-BINDING RADIOASSAY FOR 25-HYDROXYCHOLECALCIFEROL1Journal of Clinical Endocrinology & Metabolism, 1971
- 25-Hydroxycholecalciferol: Stimulation of Bone Resorption in Tissue CultureScience, 1969
- Bone Cells: Biochemical and Biological Studies after Enzymatic IsolationScience, 1964