TNFα receptor expression in rat cardiac myocytes: TNFα inhibition of L‐type Ca2+ current and Ca2+ transients
- 27 November 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 376 (1-2) , 24-30
- https://doi.org/10.1016/0014-5793(95)01238-5
Abstract
Tumor necrosis factor-α (TNFα) is a potentially powerful anti-neoplastic agent; however, its therapeutic usefulness is limited by its cardiotoxic and negative inotropic effects. Accordingly, studies were undertaken to gain a better understanding of the mechanisms of TNFα-mediated cardiodepression. Single cell RT-PCR, [125I]TNFα ligand binding and Western immunoblotting experiments demonstrated that rat cardiac cells predominantly express type I TNFα receptors (TNFRI or p60). TNFα inhibited cardiac L-type Ca2+ channel current (ICa) and contractile Ca2+ transients. Thus, it is possible that the negative inotropic effects of TNFα are the result of TNFRI-mediated blockade of cardiac excitation-contraction couplingKeywords
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