DETERMINATION OF CORTICOTROPIN (ACTH) IN HUMAN BLOOD AND URINE BY A MODIFIED OXYCELLULOSE METHOD

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Abstract
THE method of Sydnor and Sayers (1) for the determination of corticotropin (ACTH) in blood has been modified and shortened so that the entire procedure of concentrating corticotropin in an injectable volume can be completed within ten hours. With this new modification we have been able to inject the equivalent of 200 ml. of blood into a single test rat. The largest volume of blood which could be used by Sydnor and Sayers was 40 ml.; and in normal subjects, corticotropin was undetectable in this amount (2, 3). By decreasing the amount of acetic acid mixed with blood and centrifuging the mixture before adding oxycellulose, we have succeeded in removing toxic material which precluded the injection of volumes larger than 40 ml. equivalent. The modification is based on the technic developed by Takahashi for the determination of MSH in blood (4). The oxycellulose technic has also been applied to urine, so that the equivalent of a 24-hour urine sample can be tested in a single rat.

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