PREPARATION AND PROPERTIES OF ALEXINATED INTERMEDIATES THAT REACT WITH CONGLUTININ .2. EQUINE RABBIT AND HUMAN COMPLEMENT

  • 1 January 1966
    • journal article
    • research article
    • Vol. 11  (3) , 255-+
Abstract
Methods for preparing nonlytic alexinated intermediates that react with conglutinin are described for rabbit and human complements as well as equine. Certain properties of these intermediates including, particularly, their stability, are described. It has been found that, while such intermediates can be formed from the various complements, no single method works for all of them. Differences between ''hemolytic'' and ''conglutinating'' complements are believed to reflect quantitative differences in reaction kinetics and in relative proportions of various components rather than any qualitative differences in components or reaction sequence. By the use of the rabbit alexinated intermediate it has been possible to show autoreactivity of auto-stimulated rabbit immunoconglutinins.