DIRECT COMPARISON IN DOGS OF PLASMA VOLUME MEASURED WITH T-1824 AND WITH ANTIGENS

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Abstract
By means of simultaneous measurements it is found that the volume of distribution of T-1824 is identical with that of bovine albumin, bovine globulin and of the polysaccharide SHI (correlation coefficient 0.954). It can be stated with considerable certainty that: initial rapid loss of significant amts. of T-1824 by staining or phagocytosis does not occur; conventional extrapolation of the disappearance curve on a semilog plot corrects for the amt. of dye lost during the mixing period; and the volume distribution of T-1824 is a true measure of the plasma volume. In vitro tests with T-1824 and antigen show that the true relative cell volume is on the average 3.5-4% less than the hematocrit value obtained by centrifugation for 30 min. at 1500 x g.
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