An accurate method for measurement of aldosterone production

Abstract
A method is described for isolation of the acid-hydrolysable metabolite of aldosterone in sufficient purity to measure accurately the daily production rate. Values obtained with six hospital patients were 84–131μg./day on a daily intake of 100m-equiv. of Na+ and 227–464μg./day on a daily intake of 10m-equiv. of Na+. Corresponding values for aldosterone excretion were also recorded, but these are a poor index of production rate since they represent from 1·6 to 9·8% of the total daily output of aldosterone.