Electrophoretic Studies of Bovine Serum. IV. Differences in Serum Glycoproteins Due to Age and Sex.

Publisher Website
Google Scholar
Abstract
Serum proteins from 267 dairy animals from various age groups and sex were separated by paper electrophoresis. The carbohydrates bound to each fraction were stained using periodic acid Schiff reagent. Total glycoprotein content of serum was analyzed using the tryptophan reaction. Statistically significant differences occurred in the relative and absolute concentrations of the glycoproteins. Relative concentrations among age groups showed more beta and gamma fractions and less alpha fraction in the older animals than in the younger animals. Differences did not occur in the albumin fraction. Absolute concentrations showed less beta and gamma fractions and less total glycoprotein in the younger animals than in the older animals. Significant differences did not occur in the albumin and alpha fractions. Analyses among sex showed that the relative concentrations of alpha fraction was less and the beta fraction was more in the males than the females. Differences in the albumin and gamma fractions were not observed. Absolute concentrations showed less alpha but more beta and gamma fractions in the males than the females. Differences in the albumin fraction and total glycoprotein were not observed.