Effect of prolonged hypoxia on cerebrospinal fluid and plasma activity of GOT and LDH

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Abstract
The effect of prolonged hypoxia on cerebrospinal fluid and plasma GOT (glutamic-oxalacetate transaminase) and LDH (lactic dehydrogenase) activity was studied in 37 puppies. The average activity of cerebrospinal fluid GOT was 5 times greater in the hypoxic puppies than in the controls; the LDH activity was 3 times greater. Negligible increases in plasma activity of both enzymes were observed. Cisternal fluid radioactivity plasma radioactivity concentration ratios of albumin I131 were measured concomitantly with the enzyme determinations and demonstrated increased permeability of the blood-cerebrospinal fluid barrier to albumin. Cisternal fluid: plasma GOT and LDH concentration ratios were also definitely increased in the hypoxic series, suggesting that there is increased permeability of the blood-cerebrospinal fluid barrier to these enzymes after prolonged hypoxia rather than actual cerebral cell necrosis. Increased cerebral cell membrane permeability may be an additional factor.