Tracking Individual Kinesin Motors in Living Cells Using Single Quantum-Dot Imaging
Top Cited Papers
- 23 June 2006
- journal article
- Published by American Chemical Society (ACS) in Nano Letters
- Vol. 6 (7) , 1491-1495
- https://doi.org/10.1021/nl060921t
Abstract
We report a simple method using semiconductor quantum dots (QDs) to track the motion of intracellular proteins with a high sensitivity. We characterized the in vivo motion of individual QD-tagged kinesin motors in living HeLa cells. Single-molecule measurements provided important parameters of the motor, such as its velocity and processivity, as well as an estimate of the force necessary to carry a QD. Our measurements demonstrate the importance of single-molecule experiments in the investigation of intracellular transport as well as the potential of single quantum-dot imaging for the study of important processes such as cellular trafficking, cell polarization, and division.Keywords
This publication has 24 references indexed in Scilit:
- Observation of Individual Microtubule Motor Steps in Living Cells with Endocytosed Quantum DotsThe Journal of Physical Chemistry B, 2005
- Quantum Dots for Live Cells, in Vivo Imaging, and DiagnosticsScience, 2005
- Dynamics of Single mRNPs in Nuclei of Living CellsScience, 2004
- Diffusion Dynamics of Glycine Receptors Revealed by Single-Quantum Dot TrackingScience, 2003
- Kinesin motion in the absence of external forces characterized by interference total internal reflection microscopyPhysical Review E, 2003
- Organization and Dynamics of Growing Microtubule Plus Ends during Early MitosisMolecular Biology of the Cell, 2003
- Real-Time Single-Molecule Imaging of the Infection Pathway of an Adeno-Associated VirusScience, 2001
- Blinking statistics in single semiconductor nanocrystal quantum dotsPhysical Review B, 2001
- Direct observation of kinesin stepping by optical trapping interferometryNature, 1993
- Introduction of macromolecules into cultured mammalian cells by osmotic lysis of pinocytic vesiclesCell, 1982