Isolation and Characterization of Streptomyces venezuelae Mutants Blocked in Chloramphenicol Biosynthesis

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Abstract
Twelve S. venezuelae mutants blocked in chloramphenicol biosynthesis were isolated. Two of these (Cml-1 and Cml-12) were apparently blocked in the conversion of chorismic acid to p-aminophenylalanine and 3 (Cml-4, Cml-5 and Cml-8) accumulated p-aminophenylalanine and may have been blocked in the hydroxylation reaction that converted this intermediate to p-aminophenylserine. One mutant (Cml-2) accumulated D-threo-1-p-nitrophenyl-2-propionamido-1,3-propanediol and D-threo-1-p-nitrophenyl-2-isobutyramido-1,3-propanediol, indicating that chlorination of the .alpha.-N-acyl group of chloramphenicol was blocked. The remaining 6 strains did not excrete any detectable chloramphenicol pathway intermediates.