ISO‐DALT characterization of 12 ‘new’ equine plasma protease inhibitor (Pi) alleles
- 24 April 1987
- journal article
- research article
- Published by Wiley in Animal Genetics
- Vol. 18 (2) , 167-180
- https://doi.org/10.1111/j.1365-2052.1987.tb00756.x
Abstract
Twelve equine protease inhibitory alleles, PiE,H,J,K,L2,O,P,Q,R,V,X,Z, have been characterized in terms of isoelectric point, molecular mass and inhibitory activity to bovine trypsin and chymotrypsin by ISO-DALT electrophoresis. Protein maps for 20 Pi alleles including those of the eight ''Thoroughbred'' alleles (PiF,G,I,L,N,S1,S2,U) have now been determined. Five pairs of alleles,S1/S2,G/K,L/L2, P/R and U/Z, possessed varying numbers of common proteins ranging from one protein in the case of G/K and L/L2 to six in the case of U/Z. Based on these results and studies of the abnormal expressions of PiF, PiL and PiS1, a theory of at least three closely linked loci has been postulated to account for the marked heterogeneity of the equine protease inhibitory system.Keywords
This publication has 19 references indexed in Scilit:
- Genetic polymorphism and close linkage of two α1-protease inhibitors in horse serumAnimal Blood Groups and Biochemical Genetics, 2009
- Protease inhibitor system in horses: classification and detection of a new alleleAnimal Blood Groups and Biochemical Genetics, 2009
- Characterisation of the α1-protease inhibitor system in Thoroughbred horse plasma by horizontal two-dimensional (ISO-DALT) electrophoresis. 1. Protein stainingAnimal Blood Groups and Biochemical Genetics, 2009
- Frequencies of plasma protease inhibitor alleles in Australian horse breeds and the recognition of two new allelesAnimal Genetics, 1987
- Horse α-1 protease inhibitors: Relationship between the slow (S) and fast (F) isoformsInternational Journal of Biochemistry, 1985
- The plasma protease inhibitor system (Pi) of Standardbred horsesAnimal Blood Groups and Biochemical Genetics, 1984
- Sequence homology between human .alpha.1-antichymotrypsin, .alpha.1-antitrypsin, and antithrombin IIIBiochemistry, 1983
- Characterisation of the α1‐protease inhibitor system in Thoroughbred horse plasma by horizontal two‐dimensional (ISO‐DALT) electrophoresis. 2. Protease inhibitionAnimal Blood Groups and Biochemical Genetics, 1983
- Determination of molecular weights and Stokes' radii of non‐denatured proteins by polyacrylamide gradient gel electrophoresis. 2. Determination of the size of stable and labile molecular weight variants of enzymes from plant sourcesElectrophoresis, 1982
- Characterization of human, bovine, and horse antithrombin IIIBiochemistry, 1976