RNA splicing mutation in an aberrantly rearranged immunoglobulin lambda I gene.

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Abstract
The mouse cell line MOPC 315 is an IgA (.lambda.II)-producing myeloma. A derivative of MOPC 315 was studied that secretes normal .lambda.II chains but no H chain. This derivative, MOPC 315-26, contained a rearranged .lambda.I gene in addition to a rearranged .lambda.II gene. The rearranged .lambda.I gene was cloned into bacteriophage .lambda. DNA and its structure was studied. The .lambda.I gene arose by an aberrant recombination event that resulted in a single base insertion at the site of V-J region joining. The gene contained numerous point mutations in the vicinity of the junction of the V and J regions. Two point mutations occurred in the donor splice sequence normally used for the removal of the intron between the J and C regions, suggesting that the RNA synthesized from the aberrantly rearranged .lambda.I gene would be unable to undergo proper RNA splicing.