A study of enzymes that can break down tobacco-leaf components. 3. Fungal polygalacturonase on leaf fibre

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Abstract
Tobacco -leaf fiber incubated at pH 4.5 several times with fresh lots of purified polygalacturonase lost about half of its polyuronide. Milling the fiber increased the rate of release of carbohydrate from fiber with polygalacturonase and also increased slightly the total amt. liberated. Decalcification of the fiber by cation exchange with concentrated salt solns., and by extraction with acid, increased the rate of liberation of carbohydrate by polygalacturonase , up to 95% of the polyuronide present being released. Greater NaCl concns. than 0.05 [image] inhibited the action of polygalacturonase on fiber, but concns. less than 0.01 [image] activated. CaCl2 was inhibitory, except in concns. below about 0.001 [image] when using decalcified fiber.