THE IMMUNE RESPONSE OF ALLOPHENIC MICE TO THE SYNTHETIC POLYMER GLØ*: III. ALLOTYPE ANALYSIS OF ANTI‐GLØ ANTIBODY

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Abstract
Allophenic mice were produced by aggregating 8-cell embryos from a low responder (CBA) and a high responder (DBA/1) strain to the synthetic amino acid polymer GL.vphi.. In some strains of mice, the immune response to this polymer is controlled by 2 genes, .alpha. and .beta., which map to the IE/C and IA subregions of the H-2 complex, respectively. The CBA strain is .alpha. + .beta..sbd.whereas the DBA/1 strain can be considered to be .alpha. + .beta.. These 2 strains also differ in Ig allotype: CBA is allotype a and DBA/1 is allotype c. Eight CBA .tautm. DBA/1 allophenic mice were immunized with GL.vphi.. Four of the mice gave a high secondary response to the polymer. The allotype of the antibody from these mice was determined by affinity chromatography, and it was found that only allotype c was present. This means that all of the antibody was produced by high responder DBA/1 cells. There is no evidence for the cooperation of histoincompatible cells in the mounting of an immune response by allophenic mice.