A Rapid Separation of Ten Carotenoids, Three Retinoids, Alpha-Tocopherol and d-Alpha-Tocopherol Acetate by High Performance Liquid Chromatography and its Application to Serum and Vegetable Samples

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Abstract
The methods described herein were developed for the quantitative analysis of 10 carotenoids (lutein, zeaxanthin, canthaxanthin, beta-apo-8′carotenal, beta-cryptoxanthin, echinenone, lycopene, gamma-carotene, alpha-carotene, beta-carotene/15-cis-beta-carotene), 3 retinoids (retinol, retinyl acetate, retinyl palmitate) and alpha-tocopherol and d-alpha-tocopherol acetate with two-channel detection and a single sample preparation. Two fast and simple methods are described using a Spheri-5-RP-18 column, with two different mobile phases-acetonitrile:dichloromethane:methanol (70:20:10) in 10 minutes and acetonitrile:methanol (85:15) in 25 minutes. These methods would be beneficial to food scientists, cancer researchers and epidemiologists for the accurate estimation of vitamin A activity and the antioxidant capability of different compounds present in the human diet and the levels of these compounds in human serum.