MOLECULAR GENETIC ANALYSES OF THE MAJOR HISTOCOMPATIBILITY COMPLEX IN PIG FAMILIES AND RECOMBINANTS

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Abstract
Five HLA probes, one corresponding to class I genes, two corresponding to distinct class II light chain genes, DR.beta. and DQ.beta., and two to class II heavy chain genes, DR.alpha. and DQ.alpha., were used to analyse the genomic DNA of the pig. Three imformative SLA typed families and four SLA recombinants were studied by Southern blot analysis. About 16 restriction fragments, generated by EcoR1 or Hind III endonucleases, were revealed for each individual, either with the class I probe or the DR.beta. probe. The number of restriction fragments which hybridized with other probes was generally lower. Several restriction fragment-length polymorphisms were found and these segregated with SLA haplotypes. The studies on SLA recombinants showed that SLA DR.BETA.- and DQ.alpha.-like genes are probably tightly clustered within the SLA-D-MLR region.