Anticonvulsant Drugs Determined by EMIT® in Spiked Brain Samples: A Correlation with HPLC Values

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Abstract
Phenobarbital (PB), diphenylhydantoin (PHT) and carbamazepine (CBZ) levels were determined in spiked rat brain homogenates by the EMIT [enzyme multiplied immunoassay technique] assay, and these were compared with results obtained by high-pressure liquid chromatography (HPLC). The extraction procedure of the 3 drugs was identical for both methods, with minor modifications for EMIT (no addition of internal standard; reconstitution with the buffer used in routine plasma level analysis). Correlation coefficients between the data obtained by EMIT and HPLC were extremely good (PB, 0.90-0.98; PHT, 0.92-0.98; CBZ, 0.93-0.99). The EMIT system can detect PB, PHT and CBZ in brain tissue with good accuracy, although HPLC remains the most accurate and proven method available for research purposes.