Effect of Beta-Aminopropionitrile on Incorporation of Glycine by Croton Oil Pouches in Rats.

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Abstract
The influence of beta-aminopropionitrile ([beta]APN) on incorporation of glycine 1-C14 by croton oil pouches and the dermis of skin was studied in 6 control and 6 test rats. Test rats weighing 80-90 g were fed 40 mg of [beta]APN fumarate in 20 ml of water daily for 9 days. On the 8th day of pouch development each rat received 2 intraperitoneal injections of 50 [mu]c of carboxyl-labeled glycine 24 and 15 hours before sacrifice. The croton oil pouch wall and the dermis were analyzed for incorporation of glycine by the protein and the nucleic acids. Incorporation of glycine into protein residues of fibrous tissue from croton oil pouches was decreased about 25% in rats pretreated with [beta]APN. Analysis of the nucleic acid fraction indicated that no change occurs in glycine uptake in fibrous tissue of dermis or croton oil pouch in [beta]APN-treated animals as compared to controls. Results suggest that [beta]APN primarily affects glycine incorporation into collagen or ground substance rather than the nuclear portion of connective tissue.