Direct sequencing and bidirectional allelle specific polymerase chain reaction of the bovine β‐casein B variant
- 1 December 1992
- journal article
- Published by Wiley in Animal Genetics
- Vol. 23 (6) , 561-566
- https://doi.org/10.1111/j.1365-2052.1992.tb00180.x
Abstract
We have used the polymerase chain reaction (PCR) to amplify exon VII of the bovine beta-casein gene. The mutations responsible for the B variant were identified by direct sequencing of the amplification products. A bidirectional allele-specific PCR method (BAS-PCR) has been developed using oligonucleotides overlapping the mutation site at their 3' ends. This new procedure allows a rapid and reliable discrimination between the B and non-B alleles of beta-casein.Keywords
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