Immunoassay Using a Metal-complex Compound as a Chemiluminescent Catalyst. V. Continuous Immunoassay by the Use of CLCCIA

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Abstract
Immunoassay, using as a labeling reagent a synthesized metal-complex compound having catalytic activity for a chemiluminescence reaction, was continuously carried out by the sandwich method. The time necessary for all the processes (including: an immune reaction, B/F separation, a sandwich reaction, and a chemiluminescence reaction) was reduced to 50 min. Human serum albumin in the range of 5 ng to 1 μg could be determined (continuously) up to 5 times by the use of the same column (immobilized with an antibody).