INFLUENCE OF SOME SYNTHETIC ANTIOXIDANTS ON THE GROWTH AND METASTASES FORMATION OF LEWIS LUNG-CARCINOMA AND AMELANOTIC B-16 MELANOMA IN C57BL MICE

  • 1 January 1981
    • journal article
    • research article
    • Vol. 51  (5) , 379-385
Abstract
Synthetic antioxidants EOP (p-ethoxyphenol), PHP (p-hydroxypropiophenone) and DPPD (N,N''-diphenyl-p-phenylenediamine) dissolved in dimethylsulfoxide were administered s.c. to mice after s.c. implantation of Lewis lung carcinoma or amelanotic B16 melanoma into the tails. The doses of antioxidants used were 50, 100 and 200 mg/kg per day, respectively. The animals received 5 injections/wk for 2 wk. The tails with Lewis lung carcinoma were amputated after 16 days and with amelanotic melanoma after 20 days; tumor weight was measured. Mice were sacrificed 25 days after Lewis lung carcinoma and 42 days after B16 melanoma implantation, lungs were weighed and the incidence of pulmonary and extrapulmonary metastases were estimated. There was a significant increase in weight of amelanotic melanoma in animals treated with EOP or PHP (P .ltoreq. 0.05). No influence of antioxidants on the metastases incidence of B16 melanoma was found. Antioxidants used did not influence the Lewis lung carcinoma weight, whereas EOP or PHP increased the incidence of extrapulmonary metastases (P = 0.05 and P = 0.1, respectively).