RADIATION LEUKEMIA VIRUS AND ITS EFFECT ON H‐2 GENE EXPRESSION

Publisher Website
Google Scholar
Abstract
SUMMARY: In this report we demonstrate that lowered expression of the H‐2 antigens on RadLV‐induced tumour cells is a result of depressed levels of stable mRNA in these cells. Whether this observation is a result of lowered transcription or of mRNA instability is under investigation. In an effort to determine which viral sequences are essential for mediating both the H‐2 regulatory function and the transforming function of RadLV, we have begun to assemble newly integrated proviral genomes from tumours. The restriction enzyme cleavage sites of four isolates are presented; these isolates differ substantially from RadLV genomes previously presented. One of these molecular clones is shown to encode a non‐defective B‐tropic, ecotropic virus which when reinjected into resistant mouse strains can mediate the up‐regulation of H‐2Dd antigen expression. Finally, possible mechanisms of H‐2 regulation are discussed.