Photocontrol of Anthocyanin Synthesis in Milo Seedlings

Abstract
Anthocyanin synthesis in seedlings of Wheatland milo is controlled by 2 photoreactions. The 1st reaction requires more energy than the 2nd and exhibits a maximum response at about 470 m[mu]. Alternate light and dark periods induce formation of more anthocyanin than continuous light of the same total energy indicating that the 1st photoreactlon may be substrate limited. After the 1st photoreaction runs for several hours, anthocyanin formation is further controlled by a 2d photoreactlon. An exposure to far-red-radiant energy following the 1st photoreaction inhibits anthocyanin formation. The inhibitory effect of the far red is reversed by a subsequent exposure to red. Action spectra for the 2nd photoreaction are typical of those obtained from other phytochrome controlled responses.