Comparative Growth Cycles of Endamoeba Histolytica with Different Combinations of Bacteria

Publisher Website
Google Scholar
Abstract
Summary 1. The mixed bacterial flora accompanying our culture of the NRS strain of E. histolytica was analyzed into its components: Escherichia coli communior (NRS-I), E. coli anaerogenes (NRS-IA), and an unnamed paracolon bacillus (NRS-II). 2. A series of monobacterial cultures were established by chemical treatment of cysts with 1:50,000 mercuric chloride for 90 minutes at 4°C. 3. E. histolytica was established separately with the following bacteria in aqueous eggyolk infusion: NRS-II, NRS-I, Aerobacter aerogenes, E. coli communis, Proteus vulgaris, Aerobacter cloacae, Kurthia zenkeri, Streptococcus equinus, S. faecalis, and S. liquefaciens. 4. Populations of bacteria and amebas were plotted in the form of growth curves for the first six types listed above, with the relative growth response of the amebas in the same order as the above list. No correlation could be observed between the relative ranking of bacterial and amebic growth, and within rather wide limits no controlling influence of inoculum could be determined. 5. The highest growth response of the amebas was obtained with the original mixed flora.