The structure and transcription start site of major potato tuber protine gene
- 1 January 1986
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 14 (11) , 4625-4638
- https://doi.org/10.1093/nar/14.11.4625
Abstract
We have isolated recombinant lambda clones containing intact major tuber protein (patatin) genes and flanking sequences from the commercial tetraploid variety Maris Piper. The gene is composed of seven exons and six introns, spread over 4 kb of DNA. Nuclease mapping defined the 5' end of the mRNA approximately 45 bp upstream of the initiation codon. The 5' end of the gene is preceeded by a canonical TATA box sequence. The three known patatin genes encode proteins of nearly identical Mr but very different isoelectric points. The sequence of the gene does not indicate a role for patatin as one of the globulin class of plant storage proteins.Keywords
This publication has 18 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- Isolation and sequence analysis of cDNAs for the major potato tuber protein, patatinNucleic Acids Research, 1984
- Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencingGene, 1984
- Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesisGene, 1983
- Lambda replacement vectors carrying polylinker sequencesJournal of Molecular Biology, 1983
- Molecular analysis of a cell lineageNature, 1983
- Analysis of the Heterogeneity of the 40,000 Molecular Weight Tuber Glycoprotein of Potatoes by Immunological Methods and by NH2-Terminal Sequence AnalysisPlant Physiology, 1983
- Induction and Accumulation of Major Tuber Proteins of Potato in Stems and PetiolesPlant Physiology, 1983
- The end of the messageNature, 1982
- In vivo sequence requirements of the SV40 early promoter regionNature, 1981