IMMUNOLOGICAL REACTIVITY OF INSULIN IN HUMAN SERUM

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Abstract
The purpose of this study was to investigate whether a difference could be demonstrated between crystalline insulin extracted from normal human pancreas, and crystalline insulin extracted from bovine and porcine pancreas. Using Hales and Randle''s (1963) immuno-assay no immunological differences could be demonstrated between human and pig insulin. On the other hand, a significant difference was found, between pigandox insulin. An attempt was also made to determine whether an immunological difference could be demonstrated between crystalline pig insulin and crystalline human insulin from non diabetic subjects on the one hand and endogenous, circulating insulin from normal sugjects, obese subjects and diabetic subjects on the other. No such difference was found. From these experiments it is concluded that endogenous insulin in normal, obese and diabetic human sera is immunologically identical with human, crystalline insulin from non-diabetic subjects and crystalline pig insulin.