Determination of cocarboxylase in blood
- 1 April 1956
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 62 (4) , 601-605
- https://doi.org/10.1042/bj0620601
Abstract
A method for the estimation of blood cocarboxylase is described. Innovations in clude the use of Norite charcoal to obtain a blank-free apoenzyme, the addition of cyanide to the system, and the use of an internal standard during assay. Plasma showed an activating effect on cocarboxylase. Reference is made to the effects of thiamine and iodoacetate in the system. The possible significance of the sulphydryl form of cocarboxylase in decarboxylation is discussed.Keywords
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