Effectsof Gonadotrophinsin Vitroon Glucose Uptake and Lactic Acid Production of Ovaries from Prepubertal and Hypophysectomized Rats

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Abstract
The effects of varying amounts of LH [luteinizing hormone], and HCG [human chorionic gonadotropin], and FSH [follicle -stimulating hormone] on glucose uptake and lactic acid production by ovaries from prepubertal or hypophysectomized rats were studied in vitro. A stimulatory effect was obtained with LH (NTH-LH-B3) in a concentration of 0.01 [mu]g/ml medium with a dose-response relationship up to a concentration of 1 mg/ml medium. The possibility of using lactic acid production of prepubertal or hypophysectomized rat ovaries as a bio-assay method for LH is discussed. HCG added in vitro had stimulatory effects on both glucose uptake and lactic acid production of prepubertal rat ovaries. The dose-response relationship was almost indistinguishable from that of LH. FSH (NIH-FSH-S2) in high concentrations stimulated both glucose uptake and lactic acid production in ovaries from prepubertal or hypophysectomized rats. The dose-response relationship could not be explained on the basis of a recognized contamination of the FSH preparation by LH. Possible explanations for these in vitro effects of gonadotrophins on the isolated rat ovary are discussed.