Effect of Renal Ischemia on Renal Tubular Function in the Rat as Measured by PAH Uptake in Vitro

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Abstract
Using the mammalian kidney slice technique of Cross and Taggart, it was shown that renal ischemia of 15-60 minutes resulted in a greater uptake of para-aminohippuric acid (PAH) by slices from the ischemic kidney than by slices from kidneys of control animals. Renal ischemia of 2-4 hours produced a marked decrease in the amount of PAH accumulated by renal cortical slices. A 2_hour recovery period followed the ischemic period before the kidneys were removed from the rats. Complete ischemia for 1 hour (ligation of renal artery and vein as well as the capsular and ureteral vessels) without a recovery period did not produce a difference between the PAH uptake by the cortical slices of the ischemic and non-ligated kidneys in the same animal. Partial ischemia for 1 hour or less (renal vessels ligated) resulted in a greater accumulation of PAH in the cortical slices of the ischemic kidney than in the non-ligated kidney. Possible explanations for these results are discussed.