α-Amylase Purification and Separation from Gluco-Amylase by Affinity Chromatography on Cross-Linked Amylose(CL-Amylose)
- 1 January 1981
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 14 (17-18) , 1501-1514
- https://doi.org/10.1080/00032718108081476
Abstract
Using a CL-amylose column it is possible to purify α-amylase from other protein contaminants (e.g. albumin, haemoglobin, etc.) and from interfering exoamylases, by its specific retention on CL-amylose. α-Amylase is eluted off the column in the second step by a mixture containing 0.1 M NaCl in acetic acid 0.01 M and CaCl2 0.015 M. The recoveries of glucoamylase and α-amylase were 74% and 65% respectively. The retention capacity of the stationary phase is 0.5 mg of α-amylase/ml bed volume.Keywords
This publication has 12 references indexed in Scilit:
- Simultaneous Determination of Associated α- and Glucoamiuse Using Cross-Linked AmyloseAnalytical Letters, 1979
- Utilisation de l'amylose réticulée pour la détermination quantitative de l'α- et de la β-amylase en présence dans une préparation amylolytiqueBiochimie, 1978
- Isolation of α-amylase from human and hog pancreas and saliva by affinity chromatography on a modified substrateAnalytical and Bioanalytical Chemistry, 1978
- Purification d'α-amylases par chromatographie d'affinité sur amidon réticuléBiochimie, 1977
- The use of cross-linked amylose in establishing the α- or β-amylase activity of an amylolytic preparationBiochimie, 1976
- Affinity chromatography of cereal α-amylaseAnalytical Biochemistry, 1976
- Affinity column purification of amylases on protein inhibitors from wheat kernelJournal of Chromatography A, 1975
- Competitive affinity chromatography of wheat α‐amylaseFEBS Letters, 1975
- Action of amylolytic enzymes on a chromogenic substrateAnalytical Biochemistry, 1970
- Sur les enzymes amylolytiqucs III. La β‐amylase: dosage d'activité et contrôle de l'absence d'α‐amylaseHelvetica Chimica Acta, 1948