SUCCESSFUL AUTOLOGOUS TRANSPLANTATION WITH PERIPHERAL-BLOOD HEMATOPOIETIC-CELLS IN A PATIENT WITH ACUTE-LEUKEMIA

  • 1 May 1986
    • journal article
    • research article
    • Vol. 14  (4) , 312-315
Abstract
One patient with acute nonlymphocytic leukemia (ANLL) in remission was given intensive chemotherapy (DAR-regimen) as late intensification treatment. Seven leukaphereses were performed during the period of marrow recovery following aplasia induced by the DAT regimen. High numbers of nucleated cells (7.8 .times. 108/kg) and granulocyte-macrophage precrusors (9.5 .times. 104/kg) were collected and then cryopreserved and stored in liquid nitrogen. When he relapsed, the patient was treated with etoposide (600 mg/m2), cyclophosphamide (120 mg/kg), and total body irradiation (1000 rad), followed by the transfusion of thawed autologous leukocytes. The time to reach 0.5 .times. 109 granulocytes/liter and 50 .times. 109 platelets/liter was 16 and 35 days, respectively. This observation demonstrates that circulating hemopoietic stem cells are capable of complete hemopoietic reconstitution after marrow-ablative therapy with supralethal doses of chemoradiotherapy.