Monoclonal Antibody to Human β Interferon: Characterization and Application

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Abstract
Seven stable mouse hybridomas secreting monoclonal antibodies to human fibroblast .beta. interferon (IFN-.beta.) were isolated, all seven of which belonged to IgG1 subclass and .kappa. type. While neutralizing the antiviral activity of human fibroblast IFN-.beta., they failed to neutralize both that of human IFN-.alpha. and human IFN-.gamma.. These monoclonal antibodies neutralized the antiviral activity of human fibroblast IFN-.beta. but not that of human IFN-.alpha. and IFN-.gamma.. Two of the seven monoclonal antibodies, YSB-1 and YSB-2, showed particularly high neutralization titers in the ascitic fluid. Monoclonal antibodies were purified from cultures of hybridoma grown in a serum-free medium. The purified monoclonal antibodies, YSB-1(5 .mu.g/ml) and YSB-2(1 .mu.g/ml), neutralized IFN-.beta. from 10EU/ml to 1EU/ml. Human fibroblast IFN-.beta. was purified to 3.5 .times. 107 IU/mg protein (1.0 .times. 108 IU/mg protein in the peak fraction) by the monoclonal antibody (YSB-2) affinity column chromatography, whereas human recombinant IFN-.beta. obtained from Escherichia coli was also purified to 6.5 .times. 107 IU/mg protein (1.1 .times. 108 IU/mg protein in the peak fraction) by the monoclonal antibody (YSB-1) affinity column chromatography.