Solubilization of Adrenal Angiotensin II Receptors with a Zwitterionic Detergent
- 1 January 1983
- journal article
- research article
- Published by Taylor & Francis in Journal of Receptor Research
- Vol. 3 (1-2) , 289-299
- https://doi.org/10.3109/10799898309041942
Abstract
Active rat adrenal angiotensin II receptors have been solubilized with a zwitterionic detergent, 3 [(3-cholamidopropyl)-dimethylammonio] -1-propane sulfonate. The solubilized receptors retain a high affinity for angiotensin II (Kd = 1.9 nM) similar to the value observed in adrenal membrane particles (Kd = 1.2 nM). In addition, the binding-inhibition potency of several angiotensin II peptides is maintained unaltered, indicating a fully preserved specificity of the solubilized receptors.Keywords
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