WEAK PROMOTION OF C3H-10T1/2 CELL-TRANSFORMATION BY REPEATED TREATMENTS WITH FORMALDEHYDE

  • 1 January 1983
    • journal article
    • research article
    • Vol. 43  (7) , 3236-3239
Abstract
C3H/10T 1/2 [mouse] cells were treated with N-methyl-N''-nitro-N-nitrosoguanidine and then repeatedly exposed to formaldehyde (0.1 to 2.0 .mu.g/ml). Exposure of N-methyl-N''-nitro-N-nitrosoguanidine-initiated cultures to formaldehyde concentrations of 0.5 or 1.0 .mu.g/ml in a variety of treatment regimens resulted in focus formation in up to 9% of the treated dishes. Transformed foci were observed in 2% or less of the cultures treated with N-methyl-N''-nitro-N-nitrosoguanidine or formaldehyde alone. Formaldehyde thus appears to be only a weak tumor promoter for C3H/10T 1/2 cell transformation.