Enterocytic differentiation of the human Caco‐2 cell line is correlated with down‐regulation of fibronectin and laminin

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Abstract
Human intestinal Caco‐2 cells were used to examine the expression of fibronectin (FN) and laminin (LN) during enterocytic differentiation. Combination of immunoprecitation. Western and Northern blotting revealed that Caco‐2 cells expressed a classical FN and a variant form of LN: besides B1 and B2 chains, LN contained a 350‐kDa heavy chain instead of the 400‐kDa A chain. Throughout Caco‐2 cell differentiation, FN and LN synthesis decreased at both mRNA and protein levels. These data indicate that enterocytic differentiation involves both transcriptional and/or post‐transcriptional down‐regulation of FN and LN gene expression.