DNA-mediated gene transfer to baby hamster kidney cells.
- 31 December 1981
- journal article
- research article
- Published by Japan Society for Cell Biology in Cell Structure and Function
- Vol. 7 (3) , 193-201
- https://doi.org/10.1247/csf.7.193
Abstract
To establish the conditions for the efficient transfer of foreign genes to baby hamster kidney (BHK) cell lines, we investigated the factors that affect the efficiency of transformation of thymidine kinase deficient (tk-) BHK cells to the tk+ phenotype. Plasmid pAGO, which carries the herpes simplex virus (HSV) tk gene, was used as the donor DNA, and the frequencies of transformation of BHK and mouse L cells under various conditions were compared. Although the optimal times of exposure to DNA were almost the same, the two kinds of cells had different cell density dependencies; transformation of BHK cells occurred only when a limited number of cells were seeded prior to DNA transfer, whereas there was a high frequency of transformation with mouse L cells over a broader range of cell density. The frequency of transformation increased as the amounts of pAGO DNA increased, and a clear dose dependence was found. The efficiency of the transformation for BHK cells was about 80 tk+ transformants per 4 × 105 cells per 400 ng of pAGO DNA; about 50 times lower than the efficiency of transformation for mouse Ltk-cells.Keywords
This publication has 15 references indexed in Scilit:
- Transformation of mammalian cells with an amplifiable dominant-acting gene.Proceedings of the National Academy of Sciences, 1980
- Parameters governing the transfer of the genes for thymidine kinase and dihydrofolate reductase into mouse cells using metaphase chromosomes or DNASomatic Cell and Molecular Genetics, 1980
- Cotransfer of linked eukaryotic genes and efficient transfer of hypoxanthine phosphoribosyltransferase by DNA-mediated gene transfer.Proceedings of the National Academy of Sciences, 1980
- Cloning of the active thymidine kinase gene of herpes simplex virus type 1 in Escherichia coli K-12.Proceedings of the National Academy of Sciences, 1979
- Transformation of mammalian cells with genes from procaryotes and eucaryotesCell, 1979
- DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells.Proceedings of the National Academy of Sciences, 1979
- Premature chromosome condensation in a ts DNA-mutant of BHK cellsCell, 1978
- Transfer of purified herpes virus thymidine kinase gene to cultured mouse cellsCell, 1977
- Infection of Thymidine Kinase-Deficient BHK Cells with Polyoma VirusNature, 1966
- Deletion of thymidine kinase activity from L cells resistant to bromodeoxyuridineExperimental Cell Research, 1963