The lysine residue in the membrane-spanning domain of the beta chain is necessary for cell surface expression of the T cell antigen receptor.
Open Access
- 1 December 1988
- journal article
- research article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 168 (6) , 1971-1978
- https://doi.org/10.1084/jem.168.6.1971
Abstract
The TCR is a complex receptor composed of seven polypeptide chains consisting of a ligand-binding subunit, Ti, and a putative signal-transducing subunit, CD3. Phylogenetically conserved charged amino acid residues within the membrane-spanning domains present in all seven chains of the TCR have been proposed to be important in the association between Ti and CD3. Using a Ti beta chain-deficient mutant of the cell line Jurkat, site-directed mutagenesis and transfection of Ti beta chain cDNA was performed to assess the importance of the lysine residue at position 290 within the membrane-spanning domain of the Ti beta chain to expression of the TCR complex. These studies demonstrated that the lysine residue, and not simply conservation of either basic charge or secondary structure, is important at this position.Keywords
This publication has 24 references indexed in Scilit:
- Chimeric α 2 -,β 2 -Adrenergic Receptors: Delineation of Domains Involved in Effector Coupling and Ligand Binding SpecificityScience, 1988
- The T Cell Receptor/CD3 Complex: A Dynamic Protein EnsembleAnnual Review of Immunology, 1988
- Failure to synthesize the T Cell CD3-? chain: Structure and function of a partial T cell receptor complexCell, 1988
- Structure, Function, and Serology of the T-Cell Antigen Receptor ComplexAnnual Review of Immunology, 1987
- Specific antigen—la activation of transfected human T cells expressing murine Ti αβ —human T3 receptor complexesNature, 1987
- Transfer of specificity by murine α and β T-cell receptor genesNature, 1986
- Reconstitution of an active surface T3/T-cell antigen receptor by DNA transferNature, 1985
- Cross-linking of human T cell receptor proteins: association between the T cell idiotype β subunit and the T3 glycoprotein heavy subunitCell, 1985
- Sequence and expression of transcripts of the human T-cell receptor β- chain genesNature, 1984
- Genomic organization and sequence of T-cell receptor β-chain constant- and joining-region genesNature, 1984