Transfructosidase from Agave vera cruz Mill

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Abstract
The stem of Agave vera cruz contains a transfrucosidase which builds up glucofructosans from sucrose with simultaneous release of glucose. With incubation, small quantities of fructose are released. The enzyme has been partially purified and concentrated by precipitation with ammonium sulphate dialysis. No dialyzable cofactors seem to be involved. The enzyme acts on sucrose as the single substrate without requiring the interaction of agave fructosans or intermediates. No higher homologues are formed from raffinose, which is, however, slightly hydrolyzed during prolonged incubation with the enzyme. In-organic phosphate does not influence the enzyme action. The conditions for optimum action are: pH 5.6-5.8; temperature 37[degree] and a sucrose concentration of 30%. The enzyme is inactivated at 55[degree].