Bovine Semen Metabolism. IV. Effect of Added Hormones and Amino Acids on Fructolysis

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Abstract
A total of 46 semen samples was used to determine the effects of the addition of the hormones, epinephrine, insulin, thyroxine, and triiodothyronine, and of the sulfhydryl- containing amino acids, ergothioneine and cysteine, on fructolysis in bull semen. A control tube and 3 tubes with the selected levels of compound addition and semen were incubated at 37[degree]C in a 2.9% sodium citrate buffer, and fructose utilizations were determined at 60 and 120 minutes. Epinephrine addition (4-100 ug/ml resulted in an inhibition of fructolysis during the 0- to 60[long dash]minute periods, with a return to the control level during the 60- to 120-minute period. There was no apparent effect of insulin (4-100 ug/ml; thyroxine (0.04-100 ug/ml); triiodothyronine (0.008-0.200 ug/ml); ergothioneine (4-100 ug/ml), or cysteine (4-100 ug/ml) on fructose utilization.