EFFECT OF PYRIDOXAL ON UPTAKE OF C-14-ACTIVITY FROM LABELED ISONIAZID BY MYCOBACTERIUM TUBERCULOSIS

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Abstract
Pyridoxal reversed in vitro inhibition of M. tuberculosis H37Rv by isoniazid. No reversal was obtained with pyridoxine or pyridoxamine. An increase in uptake of C14-activity from labeled isoniazid by an isoniazid-susceptible strain of H37Rv of 100-fold was obtained in the presence of pyridoxal. Pyridoxine, pyridoxamine, hemin, [alpha]-ketoglutarate, sodium pyruvate, biotin, and diphosphopyridine nucleotide, all compounds which have been reported to reverse isoniazid inhibition, showed no appreciable influence on uptake of C14-activity from C14-isoniazid. The interaction of pyridoxal and isoniazid was unaffected by the presence or absence of tubercle bacilli.