THE METABOLISM OF ACETONE BODIES AND GLUCOSE IN VITRO AND THE EFFECT OF ANTERIOR PITUITARY EXTRACT

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Abstract
Rat liver tissue incubated in rat serum aerobically invariably produced acetone bodies, whether the rat had been previously fasted or fed. Ant. pituitary extract accentuated the production. Heating the extract markedly reduced its activity. Kidney and muscle extracts were inactive. In the presence of liver tissue the equilibrium: acetoacetic acid ^=*/9-hydroxy-butyric acid was very sensitive to changes in O2 tension. The high O2 cone, ordinarily used in tissue slice expts. seemed to cause an abnormal shift of the equilibrium to the left. Diaphragm, kidney, spleen, omentum and brain invariably utilized acetone bodies.. The rate of utilization by diaphragm and kidney was not influenced by ant. pituitary extract. Glucose was invariably produced by liver and kidney, more/unit of wt. by the kidney than by the liver. It was utilized by muscle, spleen, brain and omentum.

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