INHIBITION OF COMMON-ANTIGEN FLUORESCENCE IN GROUPING STREPTOCOCCI BY THE FLUORESCENT ANTIBODY METHOD

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Abstract
A fluorescent antibody method has been applied successfully to the identification of group A streptococci in throat cultures within 24 hr after receipt of specimens. The method eliminates non-specific fluorescence by employing a culture medium in which staphylococci will not multiply and by inhibiting non-specific fluorescence of streptococci other than group A (groups C and G) by application of an appropriate selected non-fluorescent antiserum prior to "staining" with fluorescent group A antibody.