A human IFN-β1, gene deleted of promoter sequences upstream from the TATA box is controlled post-transcriptionally by dsRNA
- 25 September 1984
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 12 (18) , 6979-6993
- https://doi.org/10.1093/nar/12.18.6979
Abstract
Induction of IFN-β1 RNA was studied in the mouse cell line SR117-21E transformed by a BPV episome containing the human IFN-β1 gene deleted of promoter sequences upstream from position −40. Nuclei isolated from these cells synthesize constitutively IFN-β1 RNA from the partially deleted promoter. The IFN-β1 RNA synthesized by nuclei of uninduced SR117-21E cells is similar to that made by nuclei of poly(rI):(rC)-induced cells, but does not accumulate and hence no IFN is produced unless the cells have been treated either by ds RNA or by cycloheximide. We conclude that the IFN-β1 gene has, in addition to the transcription control due to upstream promoter sequences, an additional post-transcriptional control acting on mRNA accumulation and linked to sequences close to the TATA box and RNA start site. Both controls are relieved by ds RNA.Keywords
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