THE MEDIATION OF TISSUE EOSINOPHILIA IN HYPERSENSITIVITY REACTIONS .5. COMPARATIVE-STUDY OF TISSUE EOSINOPHILIA IN THE SKIN-LESIONS OF LOCAL AND SYSTEMIC PASSIVE CUTANEOUS ANAPHYLACTIC REACTIONS

  • 1 January 1983
    • journal article
    • research article
    • Vol. 50  (1) , 85-91
Abstract
Tissue eosinophilia in active cutaneous anaphylaxis reactions is biphasic: the early phase (6 h) is induced by a low molecular (MW 300 daltons) factor (early ECF [eosinophil chemotactic factor]), and the delayed phase (24 h) is mediated by synergy of 2 different factors (delayed ECF-a and -b). The mediation of tissue eosinophilia in passive cutaneous anaphylactic (PCA) reaction sites is reported. Tissue eosinophilia in systemic PCA showed 2 phases with peaks at 6 and 24 h, while that in local PCA was monophasic and peaked at 12 h. A dialyzable ECF was isolated from the early stage (0-6 h) of local PCA skin sites, and another chemotactic factor (MW 15,000), sharing a common antigenicity with guinea pig serum C5 [complement component 5], from 12 h old local PCA skin sites. A different chemotactic factor with a MW of .apprx. 70,000, sharing a common antigenicity with delayed ECF-b isolated from active cutaneous anaphylactic skin lesions, was isolated from 24 h old systemic PCA skin lesions. Although the dialyzable factor was also isolated from systemic PCA skin sites, the factor from systemic PCA delayed skin sites may not contribute to delayed tissue eosinophilia, since the activity paralleled the intensity of basophil accumulation but not to that of eosinophils. Apparently tissue eosinophilia in systemic and local PCA reactions is mediated by different chemotactic factors.