Dormancy of the Barley Grain Is Correlated with Gibberellic Acid Responsiveness of the Isolated Aleurone Layer
- 1 December 1992
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 100 (4) , 1834-1839
- https://doi.org/10.1104/pp.100.4.1834
Abstract
The relationship between barley grain dormancy and gibberellic acid (GA(3)) responsiveness of aleurone layers has been investigated. Barley (Hordeum distichum L. cvs Triumph and Kristina) grains were matured under defined conditions in a phytotron. Grains of Triumph plants grown under long-day/warm conditions had lower dormancy levels than grains of plants grown under short-day/cool conditions. Aleurone layers isolated from grains of long-day Triumph plants secreted more alpha-amylase and had a higher responsiveness to GA(3) as measured by alpha-amylase secretion. Storage of the grains increased both the percentage of germination and the responsiveness of the aleurone to GA(3). Use of different sterilization methods to break dormancy confirmed the correlation between germination percentage and aleurone layer GA(3) responsiveness. The response of embryoless Triumph grains to GA(3) was lower than that of the isolated aleurone layers, suggesting a role of the starchy endosperm in regulating the GA(3) response of the aleurone layer. Grains of the cultivar Kristina harvested from short day- and long day-grown plants lacked dormancy, and their isolated aleurone layers had a similar responsiveness to GA(3) as measured by alpha-amylase secretion. The data indicate that the physiological state of the aleurone layers contributes to the percentage germination of the grains.Keywords
This publication has 7 references indexed in Scilit:
- Synthesis of Abscisic Acid-Responsive, Heat-Stable Proteins in Embryonic Axes of Dormant Wheat GrainPlant Physiology, 1990
- Hypochlorite Disinfection Influences the GA3-Induced Synthesis of α-Amylase Isozymes by Isolated Barley Aleurone LayersPlant Physiology, 1988
- ABA Levels and Sensitivity in Developing Wheat Embryos of Sprouting Resistant and Susceptible CultivarsPlant Physiology, 1987
- Two barley alpha-amylase gene families are regulated differently in aleurone cells.Journal of Biological Chemistry, 1985
- Evidence for Osmotic Regulation of Hydrolytic Enzyme Production in Germinating Barley SeedsPlant Physiology, 1971
- Inhibition of Early Steps in the Gibberellin-Activated Synthesis of α-AmylasePlant Physiology, 1967
- A Discussion on Alimentary Toxæmia; Its Sources, Consequences, and TreatmentProceedings of the Royal Society of Medicine, 1913