Lipids of the acetone-insoluble fraction from red-clover (Trifolium pratense) leaves

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Abstract
An improved fractionation procedure utilizing DEAE-cellulose-column chromatography was used to separate the constituents of the acetone-insoluble fraction from red-clover leaves. The approximate composition of the fraction was (by wt.): waxes, 23%; galactolipids, 25%; phospholipids, 52%. The phospholipid composition (molar proportions) was: phosphatidylcholine, 37%; phosphatidylglycerol, 23%; phosphatidyl-ethanolamine, 15%; phosphatidylinositol, 2%; uncharacterized acidic compounds, 13%; other unknown compounds, 10%. An unusual acid, probably a C16 unsaturated fatty acid, was found to be located preferentially in the phosphatidylglycerol fraction.